| Rank |
Title |
Times cited |
| 1 |
Protein measurement with the folin phenol reagent. |
305148 |
| 2 |
Cleavage of structural proteins during the assembly of the head of bacteriophage T4. |
213005 |
| 3 |
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. |
155530 |
| 4 |
DNA sequencing with chain-terminating inhibitors. |
65335 |
| 5 |
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. |
60397 |
| 6 |
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets：procedure and some applications. |
53349 |
| 7 |
Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density. |
46702 |
| 8 |
Density-functional thermochemistry. III. The role of exact exchange. |
46145 |
| 9 |
A simple method for the isolation and purification of total lipides from animal tissues. |
45131 |
| 10 |
Clustal W：improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. |
40289 |
| 11 |
Nonparametric estimation from incomplete observations. |
38600 |
| 12 |
Basic local alignment search tool. |
38380 |
| 13 |
A short history of SHELX. |
37978 |
| 14 |
Gapped BLAST and PSI-BLAST：A new generation of protein database search programs. |
36410 |
| 15 |
A revised medium for rapid growth and bio assays with tobacco tissue cultures. |
36132 |
| 16 |
Generalized gradient approximation made simple. |
35405 |
| 17 |
"Mini-mental state"：A practical method for grading cognitive state of patients for clinician. |
34532 |
| 18 |
A rapid method of total lipid extraction and purification. |
32131 |
| 19 |
Detection of specific sequences among DNA fragments separated by gel-electrophoresis. |
31904 |
| 20 |
The neighbor-joining method：A new method for reconstructing phylogenetic trees. |
30176 |
| 21 |
Analysis of relative gene expression data using real-time quantitative PCR and the 2（T）（-Delta Delta C）method. |
28870 |
| 22 |
Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides. |
28658 |
| 23 |
Processing of X-ray diffraction data collected in oscillation mode. |
28647 |
| 24 |
Regression models and life-tables. |
28439 |
| 25 |
Density-functional exchange-energy approximation with correct asymptotic-behavior. |
26475 |
| 26 |
Colorimetric method for determination of sugars and related substances. |
25735 |
| 27 |
Use of lead citrate at high pH as an electron-opaque stain in electron microscopy. |
24449 |
| 28 |
The CLUSTAL_X Windows interface：Flexible strategies for multiple sequence alignment aided by quality analysis tools. |
24098 |
| 29 |
Statistical methods for assessing agreement between two methods of clinical measurement. |
23826 |
| 30 |
Reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. |
23642 |
| 31 |
Isolation of biologically-active ribonucleic-acid from sources enriched in ribonuclease. |
23435 |
| 32 |
The attractions of proteins for small molecules and ions. |
23421 |
| 33 |
The moderator–mediator variable distinction in social psychological-research — conceptual, strategic, and statistical considerations. |
23356 |
| 34 |
Self-consistent equations including exchange and correlation effects. |
23059 |
| 35 |
Rapid colorimetric assay for cellular growth and survival — application to proliferation and cyto-toxicity assays. |
23011 |
| 36 |
Helical microtubules of graphitic carbon. |
22899 |
| 37 |
The colorimetric determination of phosphorus. |
22690 |
| 38 |
Disc electrophoresis — II. Method and application to human serum proteins. |
22074 |
| 39 |
Inhomogeneous electron gas. |
21931 |
| 40 |
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. |
21446 |
| 41 |
Confidence limits on phylogenies：an approach using the bootstrap |
21373 |
| 42 |
A new generation of Ca2+ indicators with greatly improved fluorescence properties |
19561 |
| 43 |
Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set. |
18856 |
| 44 |
High-resolution 2-dimensional electrophoresis of proteins. |
18489 |
| 45 |
MEGA4：Molecular Evolutionary Genetics Analysis（MEGA）software version 4.0. |
18286 |
| 46 |
Fuzzy sets. |
18203 |
| 47 |
Phase annealing in SHELX-90：direct methods for larger structures. |
17728 |
| 48 |
Clinical diagnosis of Alzheimer’s disease：Report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer’s Disease. |
17220 |
| 49 |
Special points for Brillouin-zone integrations. |
17087 |
| 50 |
Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. |
17067 |
| 51 |
The CES-D scale：a self-report depression scale for research in the general population. |
17055 |
| 52 |
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. |
17025 |
| 53 |
A rating scale for depression. |
16734 |
| 54 |
An inventory for measuring depression. |
16264 |
| 55 |
A simple method for displaying the hydropathic character of a protein. |
16059 |
| 56 |
Determination of serum proteins by means of the biuret reaction. |
16009 |
| 57 |
Maximum likelihood from incomplete data via EM algorithm. |
15993 |
| 58 |
Equation of state calculations by fast computing machines. |
15902 |
| 59 |
Controlling the false discovery rate：a practical and powerful approach to multiple testing. |
15898 |
| 60 |
Measurement of protein using bicinchoninic acid. |
15802 |
| 61 |
The assessment and analysis of handedness：the Edinburgh inventory. |
15517 |
| 62 |
Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. |
15469 |
| 63 |
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. |
15160 |
| 64 |
Multiple range and multiple F tests. |
15047 |
| 65 |
Electric field effect in atomically thin carbon films. |
15022 |
| 66 |
Tissue sulfhydryl groups. |
15019 |
| 67 |
Isolation of mononuclear cells and granulocytes from human blood. |
14934 |
| 68 |
The measurement of observer agreement for categorical data. |
14903 |
| 69 |
Crystallography & NMR system：a new software suite for macromolecular structure determination. |
14898 |
| 70 |
Gaussian-basis sets for use in correlated molecular calculations. 1. The atoms boron through neon and hydrogen. |
14617 |
| 71 |
PROCHECK：a program to check the stereochemical quality of protein structures. |
14462 |
| 72 |
The MOS 36-item short-form health survey（SF-36）：I. Conceptual framework and item selection. |
14332 |
| 73 |
A new look at statistical-model identification. |
14275 |
| 74 |
Improved M13 phage cloning vectors and host strains — nucleotide-sequences of the M13mp18 and pUC19 vectors |
14232 |
| 75 |
A comprehensive set of sequence-analysis programs for the vax. |
14226 |
| 76 |
MODELTEST：Testing the model of DNA. |
14099 |
| 77 |
From ultrasoft pseudopotentials to the projector augmented-wave method. |
14049 |
| 78 |
Use of avidin-biotin-peroxidase complex（ABC）in immunoperoxidase techniques：a comparison between ABC and unlabeled antibody（PAP）procedures. |
13881 |
| 79 |
Comparison of simple potential functions for simulating liquid water. |
13774 |
| 80 |
The development and use of quantum-mechanical molecular-models. 76. AM1：a new general-purpose quantum-mechanical molecular-model |
13718 |
| 81 |
Phosphorus assay in column chromatography. |
13523 |
| 82 |
MOLSCRIPT：a program to produce both detailed and schematic plots of protein structures. |
13496 |
| 83 |
Van der Waals volumes and radii. |
13417 |
| 84 |
A new and rapid colorimetric determination of acetylcholinesterase activity. |
13332 |
| 85 |
Projector augmented-wave method. |
13330 |
| 86 |
Optimization by simulated annealing. |
13293 |
| 87 |
Nitric oxide：physiology, pathophysiology, and pharmacology. |
13267 |
| 88 |
An algorithm for least-squares estimation of nonlinear parameters. |
13258 |
| 89 |
Efficiency of ab initio total energy calculations for metals and semiconductors using a plane-wave basis set. |
13084 |
| 90 |
A low-cost, high-efficiency solar-cell based on dye-sensitized colloidal TiO2 films. |
12873 |
| 91 |
A low-viscosity epoxy resin embedding medium for electron microscopy. |
12807 |
| 92 |
The Protein Data Bank. |
12754 |
| 93 |
Accurate and simple analytic representation of the electron-gas correlation-energy |
12748 |
| 94 |
Rapid alkaline extraction procedure for screening recombinant plasmid DNA. |
12721 |
| 95 |
Improved methods for building protein models in electron-density maps and the location of errors in these models. |
12649 |
| 96 |
Accurate spin-dependent electron liquid correlation energies for local spin-density calculations — a critical analysis. |
12583 |
| 97 |
Continuous cultures of fused cells secreting antibody of predefined specificity. |
12391 |
| 98 |
Homeostasis model assessment：insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. |
12257 |
| 99 |
Adsorption of gases in multimolecular layers. |
12252 |
| 100 |
MrBayes 3：Bayesian phylogenetic inference under mixed models |
12209 |
| 101 |
Atherosclerosis — an inflammatory disease. |
12119 |
